Laboratory Biologist Research Engineer

Job title: Laboratory Biologist Research Engineer

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Location: Reims, Marne

Job Description: Offer DescriptionBAP A “Life, Earth and Environmental Sciences” – Biology and Health, Life and Earth Sciences – Research Engineer
A1A43 – Laboratory Biologist Engineer, 1 year renewableThe Inserm UMR-1250 P3Cell unit in Reims is a partner in the ANR-22-CE44-0024 project “IRon Uptake Pathways in Pseudomonas aeruginosa: phenotypic switches and mechanism of regulation- IRUPP”.One of the main obstacles to the development of new antibiotics is the ability of active molecules to penetrate bacterial cells. Iron import pathways are often proposed to import antibiotics into bacteria, thus circumventing the low permeability of the bacterial membrane.
To gain access to iron, an essential nutrient, bacteria synthesize siderophores. Siderophores are released into the bacterial environment, where they capture iron before being reabsorbed by the bacteria. Antibiotics can be attached to siderophores, so when the pathogen absorbs the ferri-siderophore, it imports the antibiotic.As part of IRUPP, the Inserm UMR-1250 P3Cell unit aims to study the plasticity of expression of different iron uptake pathways during infection of the human airway epithelium by P. aeruginosa.Profile: Research engineer, 1 year renewable, starting January 1, 20251- Mission
To design, develop, adapt and perform bacterial infection experiments on an ex vivo model of human airway epithelium.2. Professional activity family
Biology and health, life and earth sciences.3. Statutory correspondence
Research engineer.4. REME professional activity family
Higher education – Research.5. REME job type
Engineer.6. Principal activities
– Design the development and carry out the specialist testing of bacterial infections of human airway epithelia obtained from primary cells cultured in an air-liquid interface.

• Bacterial cultures to prepare infecting bacterial inocula (L2).
• Primary cell cultures to obtain the ex vivo model of human airway epithelium.
• Experimental infection monitoring and time-lapse microscopy.

– Infectious waste management.
– Advise on technical options within the framework of the project; evaluate and validate
experimental choices.
– Apply safety rules (L2).
– Analyze, interpret and validate results.7. Special working conditions
– Variable working hours.
– Work in L2 laboratory.8. Main skills
Knowledge
– Cell culture.
– Conventional bacteriology.
– Applied computer scienceOperational skills
– Cell culture (lines and/or primaries).
– Bacterial plating and cultureBehavioral skills
– Analytical and critical thinking skills.
– Organizational skills.
– Interpersonal skills.Diploma required – Professional training desirable
PhD, engineering degree.
Preferred field of training: biology.Contact UMR-S 1250 P3Cell
Secretariat of Professor Thomas Guillard
+33 (0)3 26 78 77 02Where to apply E-mailtguillard@chu-reims.frRequirementsResearch Field Biological sciences » Biology Education Level PhD or equivalentSkills/Qualifications8. Main skills
Knowledge
– Cell culture.
– Conventional bacteriology.
– Applied computer scienceOperational skills
– Cell culture (lines and/or primaries).
– Bacterial plating and cultureBehavioral skills
– Analytical and critical thinking skills.
– Organizational skills.
– Interpersonal skills.Diploma required – Professional training desirable
PhD, engineering degree.
Preferred field of training: biology.Internal Application form(s) neededFiche de poste IR_U1250_IRUPP_v3_english.pdfEnglish(172.76 KB – PDF)Additional InformationWork Location(s)Number of offers available 1 Company/Institute UMR-1250 P3Cell Country France City REIMS Postal Code 51100 Street 45 RUE COGNACQ JAY GeofieldContact CityReims WebsiteStreet45 rue Cognacq Jay Postal Code51100STATUS: EXPIREDShare this page

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